Background: Blood stream infection cause by bacterial pathogens are common in Bangladesh and are often treated empirically. Knowledge of local antimicrobial activity profiles of the most common bacteria causing such infections are of great importance and may play a positive role in healthcare management.The aim of the study was to identify the bacterial pathogens causing bacteraemiaamong the patients of SylhetWomens Medical College,Bangladesh and to determine their antibiotic susceptibility pattern. Method:This retrospective cross-sectional study was conducted from April 2019 to January 2020. A total of 2085 blood samples were studied for the etiological and antimicrobial susceptibility pattern .The blood cultures positive isolates were identified by Versa TREK automated blood culture machine. Further identification of bacterial pathogens and their antimicrobial susceptibility test were performed using standard microbiological procedures. Result: Overall, 19.4% of the culture blood samples were positive and Gram positive bacteria were predominant throughout the study period. Staphylococci aureus was the most frequently isolated organism (55%) followed by Escherichia Coli (21%) and a high percentage of these isolates were multidrug resistant (MDR). Conclusion:This study reveals a significant prevalence of bacterial isolates in blood with multi drug resistance and it highlights the need for periodic surveillance of etiologic agent and antibiotic susceptibility to prevent further emergence and spread of resistant bacterial pathogens.